1332 Janus kinase inhibitor increases hair growth by increasing beta-catenin activity in outer root sheath cells

Alopecia areata (AA) is a common hair loss condition characterized by the acute onset of scarring loss. Recently, JAK inhibitors have been used to treat patients with severe AA who do not respond conventional treatments such as glucocorticosteroids, cyclosporine, and diphenylcyclopropenone. Our previous studies showed an alternative effect on follicle cells, decreasing mitochondrial damage inflammasome activation, thereby improving AA's inflammatory status. However, no reports investigated role in direct growth promotion cells. Therefore, this study β-catenin activation human primary outer root sheath (ORS) IFNγ-treatment decreased increased Dickkopf-related protein 1 (DKK1) expression ORS cells dose-dependent manner, was both cytosol nuclear fraction. Next, we each selective inhibitor, itacitinib, CEP-33779, ritlecitinib, examine which inhibitor regulates β-catenin. Itacitinib CEP-33779 nucleus inhibited IFNγ treatment ritlecitinib slightly reversed compared other inhibitors. Moreover, itacitinib transcriptional manner. shows that JAK1 JAK2 increase activity decrease DKK1 levels, providing additional therapeutic mechanism than inhibiting JAK-STAT pathway gives more information dermatologists use AA.